Effects of nutritional enrichment on acid production from degenerated (non-solventogenic) Clostridium acetobutylicum strain M5
نویسندگان
چکیده
منابع مشابه
Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum.
The fermentation carried out by the biofuel producer Clostridium acetobutylicum is characterized by two distinct phases. Acidogenesis occurs during exponential growth and involves the rapid production of acids (acetate and butyrate). Solventogenesis initiates as cell growth slows down and involves the production of solvents (butanol, acetone, and ethanol). Using metabolomics, isotope tracers, a...
متن کاملMetabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance.
The primary alcohol/aldehyde dehydrogenase (coded by the aad gene) is responsible for butanol formation in Clostridium acetobutylicum. We complemented the non-sporulating, non-solvent-producing C. acetobutylicum M5 strain (which has lost the pSOL1 megaplasmid containing aad and the acetone-formation genes) with aad expressed from the phosphotransbutyrylase promoter and restored butanol producti...
متن کاملMetabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production.
To improve butanol selectivity, Clostridium acetobutylicum M5(pIMP1E1AB) was constructed by adhE1-ctfAB complementation of C. acetobutylicum M5, a derivative strain of C. acetobutylicum ATCC 824, which does not produce solvents due to the lack of megaplasmid pSOL1. The gene products of adhE1-ctfAB catalyze the formation of acetoacetate and ethanol/butanol with acid re-assimilation in solventoge...
متن کاملPhosphotransferase Activity in Clostridium acetobutylicum from Acidogenic and Solventogenic Phases of Growth.
Clostridium acetobutylicum cells, when energized with fructose, transported and phosphorylated the glucose analog 2-deoxyglucose by a phosphoenolpyruvate-dependent phosphotransferase (PT) system. Butanol up to 2% did not inhibit PT activity, although its chaotropic effect on the cell membrane caused cellular phosphoenolpyruvate and the 2-deoxyglucose-6-phosphate to leak out. Cells harvested fro...
متن کاملSTIMULATORY EFFECTS OF CALCIUM CARBONATE ON BUTANOL PRODUCTION BY SOLVENTOGENIC Clostridium species
Solventogenic Clostridium species are motile Gram positive anaerobic bacteria used for the fermentative production of acetone-butanol-ethanol (ABE). The addition of CaCO3 to semi-defined P2 medium was investigated to evaluate its impact on growth, amelioration of butanol toxicity, glucose utilization, and ABE production by solventogenic Clostridium species. Two Clostridium species commonly used...
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ژورنال
عنوان ژورنال: Applied Biological Chemistry
سال: 2018
ISSN: 2468-0834,2468-0842
DOI: 10.1007/s13765-018-0372-6